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Table 19.3 Tier I Tests (Screen) for Immune Suppression Using Laboratory Rodents Immunopathology Hematology: Complete blood count and differential Weights: body, spleen, thymus Histology: Spleen, thymus, lymph node IgM plaque-forming cell (PFC) response to T cell-dependent antigen (e.g., SRBC) Lymphoproliferative response: T cell mitogens (Con A and PHA) Allogeneic mixed leukocyte response (MLR) Natural killer (NK) cell activity

Fig. 15.1 Pictorial indication of self-similarity: number of connection arrivals over time for traf c class HTTP on three different time scales.

41. 42.

Note: For details on speci c assays see M. I. Luster et al., Fund Appl. Tox. 10: 2 19, 1988.

The degree of burstiness over different time scales or the extent of self-similarity can be expressed with just one single parameter, the Hurst parameter [5]. For selfsimilar processes its value is between 0.5 and 1 and the degree of self-similarity increases as the Hurst parameter approaches 1. More formally, a covariance-

system of 700 linear equations! In contrast, Theorem 10.3.2 readily suggests an ~ asymptotic approximation lB 0:0767=B2 . The approximation is presented on the loss same gure with `` '' symbols. A precise match is apparent from the gure. In fact, ~ relative error jlB lB j=lB for all computed buffers was less than 4%. loss loss loss

45. 46.

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Figure 19.3 Assessing chemicals for immunosuppressive effects. The most common approach to accomplish this goal is to inject chemical and vehicle treated mice or rats with antigen and assess the antibody response. Most often the antigen injected is sheep red blood cells (SRBC); four days later slides are made with a single cell suspension of spleen cells, sheep red blood cells, and complement immobilized in agar. Slides are incubated and spleen cells making antibody against SRBC lyse the surrounding RBCs generating plaques. Plaques are counted to determine the number of antibody forming cells. Alternatively, serum can be obtained and an ELISA assay performed to detect SRBC speci c antibody.

In this section we investigate the queueing behavior when the distribution of the arrival sequence has a bounded (truncated) support [32, 34]. This arises quite frequently in practice when the arrival process distribution has a bounded support and inside that support is nicely matched with a heavy-tailed distribution (e.g., Pareto). Our primary interest in this scenario is in its possible application to network control. More precisely, one can imagine network control procedure in which short network ows are separated from long ones. If the distribution of ows is longtailed, this procedure will yield a truncated long-tailed distribution for the short network ows. Assume that long ows are transmitted separately using virtual circuits and short ows are multiplexed together. Intuitively, it can be expected that with short (truncated) ows one can obtain better multiplexing gains than with the original ones (before the separation). These gains are quanti ed in Theorem 10.3.6, which explicitly asymptotically characterizes a unique asymptotic behavior of the queue length distribution. Informally, this distribution on the log scale resembles a stair-wave function that has steep drops at speci c buffer sizes (see Fig. 10.2). This has important design implications suggesting that negligible increases of the buffer

forming cells (AFC) in the spleen (Jerne assay) or by assessing antigen speci c antibodies in serum using an enzyme-linked immunosorbent assay (ELISA). Because the SRBC is a T-dependent antigen, T and B cells, as well as antigen presenting cells, must be functional to have a successful immunization. Suppression of this response is highly predictive of suppression of other immune function tests and also correlates well with tests that assess resistance to challenge with an infectious agent or tumor cells. The disadvantage to this test is that it usually requires a dedicated set of animals because of the antigen challenge. The most common approach has been to treat the animals for 14 to 28 days with the xenobiotic of interest, inject the antigen at the end of that exposure, and collect spleen or serum 4 to 5 days later. Unlike the tests for antibody-mediated immunity, tier 1 tests for cell-mediated immunity, and natural killer cell activity can be done ex vivo and do not require a dedicated set of animals. However, these tests focus on one cell type and are not as predictive of overall immunocompetence as the antibody assays.

47. 48. 49.

Log10(Pr[Q>x])

the use of oxygenated fuels for example, fuels containing ethanol or MTBE (methyl t-butyl ether). Industries may emit various pollutants relating to their manufacturing processes acids (sulfuric, acetic, nitric, and phosphoric), solvents and resins, gases (chlorine and ammonia), and metals (copper, lead, and zinc).

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